Product Description

Rat CYP27A1 (Cytochrome P450 27A1) Microsample ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK10011MS-48, ELK10011MS-96, ELK10011MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CYP27A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CYP27A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CYP27A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CYP27A1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: CP27, CTX, CYP27, Sterol 27-Hydroxylase, Cerebrotendinous Xanthomatosis, Vitamin D(3) 25-hydroxylase, 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase

Reactivity: Rat

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Metabolic Pathway

Assay Length: 3.5h

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

UniProt: P17178

Research Use Only