Product Description

Rat CYP7A1 (Cytochrome P450 7A1) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK7308-48, ELK7308-96, ELK7308-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CYP7A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CYP7A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CYP7A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CYP7A1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: CYP7, CYP7-A1, CYPVII, Cholesterol 7 Alpha Hydroxylase, Cytochrome P450 Family 7 Subfamily A Polypeptide 1

Reactivity: Rat

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Metabolic Pathway

Assay Length: 3.5h

Sensitivity: 0.52 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

UniProt: P18125

Research Use Only