Rat ECE1 (Endothelin Converting Enzyme 1) ELISA Kit - ELK0229

Rat ECE1 (Endothelin Converting Enzyme 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0229-48, ELK0229-96, ELK0229-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: ECE1; ECE-1

Assay Type: Sandwich

Sensitivity: 22.1 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, breast milk, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Enzyme and Kinase; Cardiovascular biology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ECE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ECE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ECE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ECE1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P42893

Research Use Only