Rat EDN2 (Endothelin 2) ELISA Kit - ELK6274

Rat EDN2 (Endothelin 2) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6274-48, ELK6274-96, ELK6274-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: ET2; PPET2; Preproendothelin-2

Assay Type: Competitive Inhibition

Sensitivity: 2.66 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Cardiovascular biology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat EDN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EDN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EDN2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P23943

Research Use Only