Product Description

Rat F12 (Coagulation Factor XII) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK10027-48, ELK10027-96, ELK10027-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat F12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat F12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F12 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: FXII, HAF, Contact Factor, Hageman Factor

Reactivity: Rat

Sample Type: Plasma

Assay Type: Sandwich

Research Areas: Metabolic Pathway, Hematology

Assay Length: 3.5h

Sensitivity: 25.2 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

UniProt: D3ZTE0

Research Use Only