Rat HINT1 (Histidine Triad Nucleotide Binding Protein 1) ELISA Kit - ELK7776

Rat HINT1 (Histidine Triad Nucleotide Binding Protein 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7776-48, ELK7776-96, ELK7776-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: HINT; PKCI1; PRKCNH1; Adenosine 5'-monophosphoramidase; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1

Assay Type: Sandwich

Sensitivity: 0.113 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HINT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HINT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HINT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HINT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P62959

Research Use Only