Product Description

Rat HMGB-1 (High Mobility Group Protein B1) Microsample ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK10272MS-48, ELK10272MS-96, ELK10272MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: HMGB1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein, HMG1, High Mobility Group Protein 1

Reactivity: Rat

Sample Type: Serum, Plasma, Tissue Homogenates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Infection Immunity

Assay Length: 3.5h

Sensitivity: 0.067 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

UniProt: P63159

Research Use Only