Rat HO1 (Heme Oxygenase 1, Decycling) ELISA Kit - ELK1477

Rat HO1 (Heme Oxygenase 1, Decycling) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1477-48, ELK1477-96, ELK1477-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: HMOX1; Hsp32; HMOX1D

Assay Type: Sandwich

Sensitivity: 0.062 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Enzyme and Kinase; Metabolic pathway; Cardiovascular biology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HO1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HO1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HO1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HO1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P06762

Research Use Only