Rat hs-CRP (High Sensitivity C Reactive Protein) Microsample ELISA Kit - ELK10185MS

Rat hs-CRP (High Sensitivity C Reactive Protein) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10185MS-48, ELK10185MS-96, ELK10185MS-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: C-RP; PTX1; Pentraxin-Related; Pentraxin 1

Assay Type: Sandwich

Sensitivity: 1.08 µg/mL

Standard: 100 µg/mL

Detection Range: 1.56-100 µg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Tumor immunity; Infection immunity; Cardiovascular biology; Rheumatology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P48199

Research Use Only