Product Description

Rat HSP-70/HSPA9 (HeatShock Protein 70) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK7669-48, ELK7669-96, ELK7669-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HSP-70/HSPA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HSP-70/HSPA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HSP-70/HSPA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HSP-70/HSPA9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: DnaK, 70 Kilodalton Heat Shock Proteins, Heat shock 70 kDa protein 1, 2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1, HSP70 2, HSP70 1A, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B, HSP70, Heat Shock Protein 70

Reactivity: Rat

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Tumor Immunity, Infection Immunity, Developmental Science

Assay Length: 3.5h

Sensitivity: 1.18 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

UniProt: P48721

Research Use Only