Product Description

Rat HSPA4 (Heat Shock 70kDa Protein 4) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11932-48, ELK11932-96, ELK11932-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: Hspa4,Heat shock 70 kDa protein 4,HSP74,Ischemia responsive 94 kDa protein,Hsph2,Irp94

Sensitivity: 0.053 ng/mL

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HSPA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HSPA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HSPA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HSPA4 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O88600

Research Use Only