Product Description

Rat HSPB1 (Heat Shock 27kDa Protein 1) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK1846-48, ELK1846-96, ELK1846-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HSPB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HSPB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HSPB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HSPB1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: HSP27, HSP-B1, CMT2F, HSP28, Hsp25, Heat Shock Protein 27, 28 kDa heat shock protein, Heat shock protein beta-1, Estrogen-regulated 24 kDa protein,

Reactivity: Rat

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Tumor Immunity, Developmental Science

Assay Length: 3.5h

Sensitivity: 0.27 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

UniProt: P42930

Research Use Only