Product Description

Rat IgE (Immunoglobulin E) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2594MS-48, ELK2594MS-96, ELK2594MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IgE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IgE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IgE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IgE in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternate Names: IGHE, Immunoglobulin Heavy Constant Epsilon, Ig epsilon chain C region

Reactivity: Rat

Sample Type: Serum, Plasma,, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Infection Immunity, Immune Molecule, Hematology, Autoimmunity

Assay Length: 3.5h

Sensitivity: 14.1 ng/mL

Standard: 2000 ng/mL

Detection Method: 31.25-2000 ng/mL

Research Use Only