Product Description

Rat IGFBP7 (Insulin Like Growth Factor Binding Protein 7) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10812-48, ELK10812-96, ELK10812-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: FSTL2, IGFBP-7v, MAC25, PSF, TAF, IGFBP-rP1, MAC25 protein, Prostacyclin-stimulating factor, PGI2-stimulating factor, Tumor-derived adhesion factor

Sensitivity: 0.52 ng/mL

Assay Type: Sandwich

Standard: 100 ng/mL

Detection Range: 1.56-100 ng/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Metabolic Pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IGFBP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IGFBP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IGFBP7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only