Product Description

Rat IL12 (Interleukin 12) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9251-48, ELK9251-96, ELK9251-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: IL-12,Interleukin 12,  IL12/P70, Interleukin-12/P70

Sensitivity: 1.33 pg/mL

Assay Type: Sandwich

Standard: 200 pg/mL

Detection Range: 3.13-200 pg/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Cytokine, Infection Immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL12 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only