Rat IL1b (Interleukin 1 Beta) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK1272MS-48, ELK1272MS-96, ELK1272MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Rat
Alternative Names: TNFRSF11B; TNFRSF11-B; OCIF; OPG; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; Osteoclastogenesis Inhibitory Factor
Assay Type: Sandwich
Sensitivity: 23.6 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum
Assay Length: 1.5h
Research Areas: Cytokine; Metabolic pathway; Bone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OPG, and the Human OPG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human OPG. After TMB substrate solution is added, only those wells that contain Human OPG and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OPG in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O00300
Research Use Only