Rat IRAK4 (Interleukin 1 Receptor Associated Kinase 4) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10780-48, ELK10780-96, ELK10780-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: NY-REN-64
Assay Type: Sandwich
Sensitivity: 0.61 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Areas: Signal transduction; Enzyme and Kinase; Apoptosis; Tumor immunity; Infection immunity; Pulmonology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IRAK4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IRAK4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IRAK4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IRAK4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only