Product Description

Rat KLK1 (Kallikrein 1) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK10629-48, ELK10629-96, ELK10629-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat KLK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KLK1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: KLKR, Klk6, HK1, Kallikrein 1, Renal/Pancreas/Salivary, Kidney/pancreas/salivary gland kallikrein, Tissue kallikrein

Reactivity: Rat

Sample Type: Serum, Plasma, other biological fluids

Assay Type: Competitive Inhibition

Research Areas: Enzyme and Kinase, Tumor Immunity, Hepatology

Assay Length: 2h

Sensitivity: 0.11 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

UniProt: P00758

Research Use Only