Rat LL-37 (Antibacterial Protein LL-37) Microsample ELISA Kit - ELK10870MS

Rat LL-37 (Antibacterial Protein LL-37) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10870MS-48, ELK10870MS-96, ELK10870MS-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic; CAMP; Cathelicidin Antimicrobial Peptide

Assay Type: Sandwich

Sensitivity: 0.24 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 3.5h

Research Areas: Infection immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LL-37 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only