Product Description

Rat MEPE (Matrix Extracellular Phosphoglycoprotein) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6848MS-48, ELK6848MS-96, ELK6848MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: OF45, Asarm Motif-Containing, Osteoblast/Osteocyte Factor 45

Sensitivity: 0.131 ng/mL

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Tumor immunity, Bone metabolism,

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MEPE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MEPE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MEPE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MEPE in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q9ES02

Research Use Only