Product Description

Rat MGMT (O-6-Methylguanine DNA Methyltransferase) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK7160-48, ELK7160-96, ELK7160-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MGMT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MGMT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MGMT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MGMT in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Methylated-DNA--Protein-Cysteine Methyltransferase, 6-O-methylguanine-DNA methyltransferase, Methylated-DNA--protein-cysteine methyltransferase

Reactivity: Rat

Sample Type: Tissue Homogenates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Enzyme and Kinase, Tumor Immunity

Assay Length: 3.5h

Sensitivity: 27 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

UniProt: P24528

Research Use Only