Product Description

Rat MT (Melatonin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0721-48, ELK0721-96, ELK0721-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: N-Acetyl-5-Methoxytryptamine

Sensitivity: 2.78 pg/mL

Assay Type: Competitive Inhibition

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Cell Lysates, Other Biological Fluids

Assay Length: 2.5h

Research Area: Endocrinology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MT in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only