Rat mTOR (Serine/threonine-protein kinase mTOR) ELISA Kit - ELK9209

Rat mTOR (Serine/threonine-protein kinase mTOR) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9209-48, ELK9209-96, ELK9209-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; FK506 Binding Protein 12 Rapamycin Associated Protein; Mammalian Target Of Rapamycin; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin and FKBP12 target

Assay Type: Sandwich

Sensitivity: 0.125 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Cell proliferation

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MTOR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MTOR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MTOR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MTOR in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P42346

Research Use Only