Rat NAP1L4 (Nucleosome Assembly Protein 1 Like Protein 4) ELISA Kit - ELK7506

Rat NAP1L4 (Nucleosome Assembly Protein 1 Like Protein 4) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7506-48, ELK7506-96, ELK7506-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: NAP2; NAP2L; hNAP2; Nucleosome assembly protein 2

Assay Type: Sandwich

Sensitivity: 0.065 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Length: 3.5h

Research Areas: Neuroscience

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NAP1L4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NAP1L4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NAP1L4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NAP1L4 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q5U2Z3

Research Use Only