Rat NES1 (Nesfatin 1) ELISA Kit - ELK4864

Rat NES1 (Nesfatin 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4864-48, ELK4864-96, ELK4864-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Nesfatin-1

Assay Type: Competitive Inhibition

Sensitivity: 183.3 pg/mL

Standard: 40000 pg/mL

Detection Range: 625-40000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Glycolipid metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NES1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NES1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NES1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only