Rat NMB (Neuromedin B) ELISA Kit - ELK4997

Rat NMB (Neuromedin B) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4997-48, ELK4997-96, ELK4997-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: NM-B

Assay Type: Competitive Inhibition

Sensitivity: 43.9 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Sample Type: Serum, plasma, other biological fluids

Assay Length: 2h

Research Areas: Signal transduction; Endocrinology; Neuroscience; Gastroenterology; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NMB in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only