Rat NPFF (Neuropeptide FF) ELISA Kit - ELK10833

Rat NPFF (Neuropeptide FF) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10833-48, ELK10833-96, ELK10833-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: FMRFAL; Neuropeptide FF-Amide Peptide Precursor; Pro-FMRFamide-related neuropeptide FF

Assay Type: Competitive Inhibition

Sensitivity: 5.9 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Neuroscience

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NPFF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NPFF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NPFF in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q9WVA9

Research Use Only