Rat NT-ProANP (N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit - ELK2400

Rat NT-ProANP (N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2400-48, ELK2400-96, ELK2400-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: NT-Pro-ANP; N-ANP

Assay Type: Sandwich

Sensitivity: 0.258 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Endocrinology; Cardiovascular biology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only