Product Description

Rat ON (Osteonectin) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK1683-48, ELK1683-96, ELK1683-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ON. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ON. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ON, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ON in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: SPARC, BM-40, Secreted Protein, Acidic, Cysteine-Rich, Basement-membrane protein 40, Secreted protein acidic and rich in cysteine

Reactivity: Rat

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Metabolic Pathway, Tumor Immunity, Infection Immunity, Hepatology, Bone Metabolism

Assay Length: 3.5h

Sensitivity: 0.249 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

UniProt: P16975

Research Use Only