Rat OPG (Osteoprotegerin) ELISA Kit - ELK10438

Rat OPG (Osteoprotegerin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10438-48, ELK10438-96, ELK10438-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: TNFRSF11B; TNFRSF11-B; OCIF; OPG; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; Osteoclastogenesis Inhibitory Factor

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Cytokine; Metabolic pathway; Bone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat OPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat OPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OPG in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O08727

Research Use Only