Product Description

Rat p-JNK (Phosphorylated c-Jun N-terminal kinases) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK0960-48, ELK0960-96, ELK0960-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-JNK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-JNK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-JNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat p-JNK in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Bsk, BSK, CG5680, D-JNK, D-junk, DBSK/JNK, dJnk, dJNK, DJNK, DJNK/bsk, Dmel\CG5680, DmJNK, dNJNK, jnk, Jnk, JNK, jnk (bsk), JNK/SAPK, Junk, p-JNK, P-JNK, pJNK, SAPKa

Reactivity: Rat

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Enzyme and Kinase

Assay Length: 3.5h

Sensitivity: 0.148 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Research Use Only