Product Description

Rat PAR4 (Protease Activated Receptor 4) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4823MS-48, ELK4823MS-96, ELK4823MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: F2RL3, PAR-4, Coagulation Factor II Thrombin Receptor-Like 3, Coagulation factor II receptor-like 3

Sensitivity: 0.118 ng/mL

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Hematology,

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PAR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PAR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PAR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PAR4 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q920E0

Research Use Only