Product Description

Rat PCSK6 (Proprotein Convertase Subtilisin/Kexin Type 6) Microsample ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK10835MS-48, ELK10835MS-96, ELK10835MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PCSK6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PCSK6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PCSK6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PCSK6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: PACE4, SPC4, Paired Basic Amino Acid Cleaving System 4, Subtilisin-Like Proprotein Convertase 4, Subtilisin/Kexin-Like Protease PACE4

Reactivity: Rat

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Enzyme and Kinase

Assay Length: 3.5h

Sensitivity: 1.26 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

UniProt: Q63415

Research Use Only