Rat PDE5A (Phosphodiesterase 5A, cGMP Specific) ELISA Kit - ELK0189

Rat PDE5A (Phosphodiesterase 5A, cGMP Specific) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0189-48, ELK0189-96, ELK0189-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CGB-PDE; cGMP-binding cGMP-specific phosphodiesterase; cGMP-specific 3',5'-cyclic phosphodiesterase

Assay Type: Sandwich

Sensitivity: 0.074 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Enzyme and Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PDE5A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PDE5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PDE5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PDE5A in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O54735

Research Use Only