Rat PGF2α (Prostaglandin F2 Alpha) ELISA Kit - ELK9878

Rat PGF2α (Prostaglandin F2 Alpha) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9878-48, ELK9878-96, ELK9878-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: PGF2A; PGF2-A; PG-F2a

Assay Type: Competitive Inhibition

Sensitivity: 3.71 pg/mL

Standard: 800 pg/mL

Detection Range: 12.5-800 pg/mL

Sample Type: Serum, plasma, saliva and other biological fluids

Assay Length: 2h

Research Areas: Metabolic pathway; Cardiovascular biology; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PGF2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only