Rat PIGF (Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit - ELK9397

Rat PIGF (Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9397-48, ELK9397-96, ELK9397-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: PHOSPHATIDYLINOSITOL GLYCAN; Phosphatidylinositol glycan biosynthesis class F protein; Phosphatidylinositol-glycan biosynthesis class F protein; PIG F; PIG-F; PIGF; PIGF_HUMAN; PIGF1

Assay Type: Competitive Inhibition

Sensitivity: 6.1 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.6-1000 ng/mL

Sample Type: Serum, plasma, other biological fluids

Assay Length: 2h

Research Areas: Signal transduction

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PIGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PIGF in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only