Product Description

Rat PIGF (Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK9397-48, ELK9397-96, ELK9397-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PIGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PIGF in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: PHOSPHATIDYLINOSITOL GLYCAN, Phosphatidylinositol glycan biosynthesis class F protein, Phosphatidylinositol-glycan biosynthesis class F protein, PIG F, PIG-F, PIGF, PIGF_HUMAN, PIGF1

Reactivity: Rat

Sample Type: Serum, Plasma, other biological fluids

Assay Type: Competitive Inhibition

Research Areas: Signal Transduction

Assay Length: 2h

Sensitivity: 6.1 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.6-1000 ng/mL

Research Use Only