Product Description

Rat pMAPT /pTAU (phosphorylated microtubule-associated protein tau) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK9247-48, ELK9247-96, ELK9247-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pMAPT /pTAU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pMAPT /pTAU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pMAPT /pTAU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat pMAPT /pTAU in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: pMAPT /pTAU

Reactivity: Rat

Sample Type: Serum, Plasma, other biological fluids

Assay Type: Sandwich

Research Areas: Intracellular Signal Transduction and Neuron'S Development and Viability.

Assay Length: 3.5h

Sensitivity: 12.64 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Research Use Only