Product Description

Rat pNF-H (Phosphorylated Neurofilament H) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6916-48, ELK6916-96, ELK6916-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: NFH, 200 kDa neurofilament protein, Neurofilament triplet H protein, NEFH, Neurofilament, Heavy Polypeptide

Sensitivity: 5.5 pg/mL

Assay Type: Sandwich

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Neuro Science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pNF-H. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pNF-H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pNF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat pNF-H in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only