Rat PRF1 (Perforin 1) ELISA Kit - ELK7298

Rat PRF1 (Perforin 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7298-48, ELK7298-96, ELK7298-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Assay Length: 3.5h

Research Areas: Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PRF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PRF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PRF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PRF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P35763

Research Use Only