Rat PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) ELISA Kit - ELK2681

Rat PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2681-48, ELK2681-96, ELK2681-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: PGG/HS; PGHS2; PHS2; HCox2; COX2; Cyclooxygenase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2; PTGS2; Prostaglandin Endoperoxide Synthase 2

Assay Type: Sandwich

Sensitivity: 0.065 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Enzyme and Kinase; Tumor immunity; Infection immunity; Reproductive science; Pulmonology; Gastroenterology; Hormone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PTGS2/COX-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PTGS2/COX-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PTGS2/COX-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PTGS2/COX-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P35355

Research Use Only