Product Description

Rat sCD163 (Soluble Cluster of Differentiation 163) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11984-48, ELK11984-96, ELK11984-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: sCD163, Soluble Cluster of Differentiation 163, M130, MM130

Sensitivity: 5.7 ng/mL

Assay Type: Sandwich

Standard: 4000 ng/mL

Detection Range: 62.5-4000 ng/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Cd And Adhesion Molecule, Metabolic Pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat sCD163. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sCD163. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat sCD163, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat sCD163 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only