Rat SCGN (Secretagogin) ELISA Kit - ELK6364

Rat SCGN (Secretagogin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6364-48, ELK6364-96, ELK6364-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: SECRET; SEGN; CALBL; Calbindin-Like; Secretagogin,EF-Hand Calcium Binding Protein

Assay Type: Competitive Inhibition

Sensitivity: 23.9 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Metabolic pathway

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat SCGN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SCGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SCGN in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q6R556

Research Use Only