Product Description

Rat SHBG (Sex Hormone Binding Globulin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1444-48, ELK1444-96, ELK1444-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: ABP, TEBG, SBP, Sex steroid-binding protein, Testis-specific androgen-binding protein, Testosterone-estradiol-binding globulin, Testosterone-estrogen-binding globulin

Sensitivity: 0.276 ng/mL

Assay Type: Sandwich

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Endocrinology, Reproductive Science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SHBG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SHBG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SHBG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SHBG in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P08689

Research Use Only