Rat SPI1 (Spleen Focus Forming Virus Proviral Integration Oncogene) ELISA Kit - ELK8056

Rat SPI1 (Spleen Focus Forming Virus Proviral Integration Oncogene) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8056-48, ELK8056-96, ELK8056-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: OF; PU.1; SFPI1; SPI-1; SPI-A; SPI-1 Proto-Oncogene; Hematopoietic Transcription Factor PU.1; 31 kDa Transforming Protein

Assay Type: Sandwich

Sensitivity: 0.114 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Length: 3.5h

Research Areas: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SPI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SPI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SPI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SPI1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only