Rat SREBP-1C (Sterol Regulatory Element-Binding Protein 1C) ELISA Kit - ELK0117

Rat SREBP-1C (Sterol Regulatory Element-Binding Protein 1C) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0117-48, ELK0117-96, ELK0117-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: ADD-1; ADD1; SREBP-1; SREBP-1c; Srebp1

Assay Type: Sandwich

Sensitivity: 18.3 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Cardiovascular; Epigenetics and Nuclear Signaling; Cancer; Metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SREBP-1C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SREBP-1C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SREBP-1C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SREBP-1C in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only