Rat SYP (Synaptophysin) ELISA Kit - ELK10871

Rat SYP (Synaptophysin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10871-48, ELK10871-96, ELK10871-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Major synaptic vesicle protein p38

Assay Type: Sandwich

Sensitivity: 10.94 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Neuroscience

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SYP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SYP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SYP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SYP in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P07825

Research Use Only