Product Description

Rat T4 (Thyroxine) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8716-48, ELK8716-96, ELK8716-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: 3,5,3',5'-Tetraiodothyronine, L-Thyroxine, Levothyroxine

Sensitivity: 1.49 ng/mL

Assay Type: Competitive Inhibition

Standard: 300 ng/mL

Detection Range: 4.69-300 ng/mL

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Length: 2h

Research Area: Endocrinology, Hormone Metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat T4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat T4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only