Product Description

Rat TRACP-5b (TartrateResistant Acid Phosphatase 5b) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK9632-48, ELK9632-96, ELK9632-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: ACP5, TRAP5b, SPENCDI

Reactivity: Rat

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Immunology, Cardiovascular

Assay Length: 3.5h

Sensitivity: 0.36 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Research Use Only