Rat TSP-1 (Thrombospondin-1) ELISA Kit - ELK10312

Rat TSP-1 (Thrombospondin-1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10312-48, ELK10312-96, ELK10312-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1

Assay Type: Sandwich

Sensitivity: 0.46 ng/mL

Standard: 100 ng/mL

Detection Range: 1.56-100 ng/mL

Sample Type: Plasma

Assay Length: 3.5h

Research Areas: Infection immunity; Hematology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q71SA3

Research Use Only