Rat Vaspin (Visceral Adipose Tissue Derived Serine Protease Inhibitor) ELISA Kit - ELK2026

Rat Vaspin (Visceral Adipose Tissue Derived Serine Protease Inhibitor) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2026-48, ELK2026-96, ELK2026-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: SERPINA12; OL-64; Visceral Adipose-Specific SERPIN; Serine Proteinase Inhibitor,Clade A Member 12; Visceral adipose-specific serpin

Assay Type: Sandwich

Sensitivity: 36.85 pg/mL

Standard: 6000 pg/mL

Detection Range: 93.75-6000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 3.5h

Research Areas: Metabolic pathway; Endocrinology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Vaspin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Vaspin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Vaspin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Vaspin in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q8R4Z1

Research Use Only